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In Vitro Probiotic Effect of Pseudostellaria heterophylla Fibrous Roots on Feed Bacillus subtilis and Preliminary Study on Its Solid Fermentation
 
     
 
时间:2020-02-03  来源:研发中心
 
 
 
      Traditional Chinese medicine Pseudostellaria heterophylla fibrous roots have been widely studied and applied in non-antibiotic clinical breeding of livestock and poultry. In vitro probiotic effect of P. heterophylla fibrous roots extract on feed probiotics Bacillus subtilis BD-K010 was studied, and the feasibility of solid fermentation of P. heterophylla fibrous roots was preliminarily evaluated. For in vitro probiotic effect, the increased concentration of P. heterophylla fibrous roots extract dependently increased the total biomass of B. subtilis BD-K010; 1.0% P. heterophylla fibrous roots extract received the best effect, and the final p H of 1.0% experimental group was closer to neutral. Meantime, B. subtilis BD-K010 with optimum concentration of P. heterophylla fibrous roots showed significantly higher in vitro antibacterial effect than the control group(P<0.01),and the antibacterial effects on Escherichia coli and Staphylococcus aureus were improved by 51.99% and 63.16%, but it was ineffective to Salmonella. For solid fermentation, the profile of substrate complex and appendage flocculent structure on substrate surface at the end of fermenta-tion in experimental groups added with B. subtilis BD-K010 and cellulase plus BD-K010 were more complex; the live bacteria number, polysaccharide content and saponin content at fermentation end-point in two experimental groups were extremely higher than those in the control group(P<0.01).P. heterophylla fibrous roots extract had good in vitro probiotic effect on B. subtilis BD-K010 and promoted its antibacterial effect, and it is feasible to use probiotics for solid fermentation of P. heterophylla fibrous roots and to improve effective components. It is of great significance to further de-velop and utilize P. heterophylla fibrous roots resources in modern animal husbandry.

   翻译:中药太子参须具有补益增免功效,在畜禽无抗养殖临床上已得到广泛研究与应用。本文试验皆在研究太子参须提取物对饲用益生菌枯草芽孢杆菌BD-K010的体外益生作用效果,并初探性考察与评价太子参须固体发酵的可行性。太子参须体外益生效果可知:较对照组,太子参须提取物添加浓度的增加能够依赖性提高菌株BD-K010的总生物量,以1.0%最优,且1.0%试验组终点pH值更接近于中性;同时太子参须优化浓度(1.0%)条件下的菌株BD-K010体外抑菌能力较对照组极显著提高(P<0.01),其对畜禽致病性指示菌大肠杆菌和金黄色葡萄球菌的抑菌效力分别提高51.99%、63.16%,但对指示菌沙门氏菌无效;太子参须固态发酵结果表明:较空白对照组,添加菌株BD-K010试验组和添加菌酶试验组的发酵终期底物复合物轮廓和基质表面附属菌体絮状结构更为复杂,两组试验组发酵终点的活菌数、多糖含量和皂苷含量分别极显著高于对照组(P<0.01)。结果表明:太子参须提取物对饲用益生菌枯草芽孢杆菌BD-K010具有良好的体外益生效果和抑菌促进功能,且利用益生菌固态发酵太子参须及提高其有效组分的技术可行,这对进一步开发太子参须资源在现代畜牧养殖业中的广泛应用具有重要意义。

 
   
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